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Registros recuperados: 122
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Test écotoxicologique sur microalgues et lecteur de plaques : mesures d'activités enzymatiques sur cellules in vivo. Application à l'évaluation de la qualité du milieu ArchiMer
Gilbert, Franck; Galgani, Francois.
A new method for the assessment of several contaminant effects on marine microalgae (Tetraselmis suesica, Skeletonema costatum) has been developped. The method is based on the measure of cells esterase activity using a fluorimetric stain, fluorescein diacetate (FDA). The experiments are performed in microplates using a fluorimetric microplate reader. The different steps of the experiments and the data processing are controlled by an original specific software. Application of the system for rapid ecotoxicological tests (determination of LC 50) and for assessment of environment quality by observation of water samples are proposed.
Tipo: Text Palavras-chave: Environnement; Écotoxicologie; CL 50; Estérase; Microalgues; FDA; Microplaques; Environment; Ecotoxicology; Le 50; Esterase; Microalgae; FDA; Microplates.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00156/26727/24808.pdf
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Substitutes for live microalgae in mariculture: a review ArchiMer
Robert, Rene; Trintignac, P.
In mollusc hatcheries and nurseries, live microalgae are traditionally used as feed for bivalves. Despite recent improvements in efficiency, such intensive culture of microalgae represents major cost factors in the production of bivalve larvae and spat. In an attempt to provide cost-effective alternatives and to simplify hatchery-nursery procedures, non-living algal food has been developed over the past few years. Substitution products such as bacteria, yeast, vitamin or docosahexaenoic acid enriched powders, dry and concentrated microalgae and microcapsules have been tested and evaluated on an experimental scale. The present article reviews major research aimed at developing non living diets in mariculture, which generally resulted in lower growth and...
Tipo: Text Palavras-chave: Mollusc rearing; Artificial feed; Microalgae; Hatchery; Nursery.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00090/20076/17719.pdf
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Microalgues marines : les enjeux de la recherche ArchiMer
Muller-feuga, Arnaud.
Industrial potentialities related to biotechnology of microalgae are examined, highlighting a wide spectrum of applications which concern principally nutrition, health, environment and energy. The french situation of research on microalgae, especially at Ifremer, is assessed, showing that the main axis are fine chemistry, aquaculture, environment, bio-process engineering. It covers the majority of the applications, but leaves in shade certain important aspects such as human food and CO2 fixation. Concerning the general approach, some significant improvements can be expected from a more accurate control of bacteriological and genetic quality of cultures. About the industrial aspects, a national initiative seems in progress. The variety of potential...
Tipo: Text Palavras-chave: Process engineering; Energy; Environment; Health; Food; Aquaculture; Biotechnology; Microalgae; Génie des procédés; Energie; Environnement; Santé; Alimentation; Aquaculture; Biotechnologie; Microalgues.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/rapport-1092.pdf
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Open-air treatment of wastewater from land-based marine fish farms in extensive and intensive systems: current technology and future perspectives ArchiMer
Hussenot, Jerome; Lefebvre, Sébastien; Brossard, Nicolas.
Marine land-based fish farms located in coastal wetlands (salt-pond zones, lagoon banks, etc.), whether extensive or intensive, send farm effluents directly to the sea or after short periods of stocking in retention reservoirs. The aims of our investigation have been to compare the efficiency of current and potential water treatment procedures in open-air. Wastewater retention ponds in commercial farms (Atlantic coasts of France) are efficient in removing up to 1 metric ton of particulate material (dry weight) per hectare and per day (faeces and unconsumed feed), but are inefficient in reducing dissolved wastes, both organic (urea, amino acids, protein) and inorganic (total ammonia nitrogen, phosphates). Forthcoming outdoor technology to treat these forms...
Tipo: Text Palavras-chave: Traitement de l'eau; Effluent; Décantation; Sédimentation; Écumage; Microalgue; Huître; Water treatment; Effluent; Aquaculture; Sedimentation; Foam fractionation; Microalgae; Oyster.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/1998/publication-873.pdf
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Does bivalve mucus favour the growth of their main food source, microalgae? ArchiMer
Cognie, B; Barille, Laurent.
Bivalves, by releasing inorganic nutrients, can stimulate the growth of the primary producers on which they feed. Dissolved excretion and mineralization of biodeposits (pseudofaeces and faeces), two indirect processes related to their feeding activity, have already been identified. This study investigated whether microalgal growth is also dependent on the mucus secreted by bivalves and rejected with their biodeposits. An experimental study based on algal growth tests was conducted to determine whether the mucus coating the pseudofaeces and faeces of the oyster Crassostrea gigas (Thunberg) could stimulate the growth of nine marine microalgae: a Prasinophyceae [Tetraselmis suecica (Butcher)] and eight Diatomophyceae [Amphora sp. (Ehrenberg), Chaetoceros sp....
Tipo: Text Palavras-chave: Bivalve; Microphyte; Mucus; Fèces; Pseudofèces; Bivalve; Microalgae; Mucus; Faeces; Pseudofaeces.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00325/43607/43926.pdf
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INFLUENCE OF DIFFERENT DIETS ON LENGTH AND BIOMASS PRODUCTION OF BRINE SHRIMP ARTEMIA FRANCISCANA (KELLOG, 1906)A. Boletín de Investigaciones
García-Ulloa Gómez,Manuel; Gamboa Delgado,Julián; Zavala Aguirre,José Luis; Ogura Fujii,Tetsuya; Lavens,Patrick.
Total length and biomass production of the brine shrimp Artemia franciscana were studied fed on soybean and wheat micropulverized meals (applied alone or mixed at different proportions), live microalgae (Tetraselmis suecica and Chaetoceros calcitrans), and dried Spirulina as diets. Eight diets were tested in triplicates during 10 days. Significant differences (P < 0.05) were observed from day 1 onwards. The mixed meal-based diets showed better production results. At day 1, the Artemia nauplii fed on the 70% wheat meal/ 30% soya meal diet were 30% longer compared to the animals from the C. calcitrans group. At day 10, the organisms fed with the 100% soya meal diet were 68% longer than those fed on the C. calcitrans diet. The final biomass production (wet...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia franciscana; Soya meal; Wheat meal; Microalgae; Length; Biomass production.
Ano: 1999 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97611999000100001
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Periwinkle (Gastropoda: Littorinidae) habitat selection and its impact upon microalgal populations BJB
APOLINÁRIO,M.; COUTINHO,R.; BAETA-NEVES,M. H..
The present study examines habitat selection and grazing pressure of the periwinkle Nodilittorina lineolata (Gmelin, 1791) on the rocky shore of Arraial do Cabo, RJ, Brazil (lat. 23°S, long. 43°W). Transfer experiments suggest that periwinkles actively select the mid intertidal, where the cirripede Chthamalus bisinuatus Pilsbry is the dominant sessile invertebrate. We also conducted a caging experiment in the middle intertidal, manipulating grazers and light, to assess the impact of grazing upon microalgal density. Grazing pressure significantly reduced microalgal abundance at the mid-intertidal level, suggesting that food availability plays an important role in the habitat selection of periwinkles on the studied shore.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Habitat selection; Intertidal; Littorinidae; Microalgae.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71081999000200005
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Phytoplankton community of a polymictic reservoir, La Plata River basin, Uruguay BJB
PÉREZ,M. C.; BONILLA,S.; MARTÍNEZ,G..
This paper deals with the analysis of phytoplankton composition and abundance from four sampling stations at the polymictic system, Rincón del Bonete water reservoir in Uruguay. Sampling data were obtained in 4 seasonal periods between February and November 1993. A hundred and twenty-four taxa were identified, where Aulacoseira granulata (Ehrenb.) Simon., A. granulata var. angustissima (Muller) Simon., A. granulata var. angustissima f. spiralis, (Muller) Simon., A. cf. ambigua, (Grun.) Simon., A. cf.. distans (Ehrenb.) Simon., Cryptomonas spp. and Synedra ulna (Nitzsch) Ehrenberg, were always present. Phytoplankton abundance fluctuated between 29 (autumn) and 2129 (summer) ind/ml. The general dominance of Aulacoseira spp. could be related to the polymictic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Freshwater; Artificial lakes; Aulacoseira; Uruguay.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71081999000400002
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The role of microalgae in aquaculture: situation and trends ArchiMer
Muller-feuga, Arnaud.
Algae are utilized diversely in aquaculture, but their main applications are related to nutrition. They are used in toto, as a sole component or as a food additive to supply basic nutrients, color the flesh of salmonids or for other biological activities. The need for nutritional sources safer than traditional animal products has renewed interest in plants in general and algae in particular. This report deals principally with the nutritional role of microalgae in aquaculture.The larvae of molluscs, echinoderms and crustaceans as well as the live prey of some fish larvae feed on microalgae. Though attempts have been made to substitute inert particles for these micro-organisms which are difficult to produce, concentrate and store, only shrimp and live prey...
Tipo: Text Palavras-chave: Shrimp; Post larvae; Phytoplankton; Molluscs; Microalgae; Hatchery; Fish; Aquaculture.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-497.pdf
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The food quality of Tetraselmis suecica slurry for Crassostrea gigas spat ArchiMer
Robert, Rene; Parisi, Guilina; Pastorelli, Roberta; Poli, Bianca Maria; Tredici, Mario.
In an attempt to provide cost-effective alternatives to live microalgae and to simplify hatchery-nursery procedures, concentrated microalgae appear to be very promising. Due to its high preservation ability, Prasinophytes appear to be good candidates for such alternative diets. Nevertheless, in a previous study the utilization of Tetraselmis suecica was only conceivable for old Crassostrea gigas larvae. In the present work its nutritional quality has been accordingly searched on ]apanese oyster's juveniles. Whatever the assemblages and condition, fresh or preserved microalgae, T. suecica did not allow an adequate postlarval development in C. gigas.
Tipo: Text Palavras-chave: Nutritional quality; Crassostrea gigas; Tetraselmis suecica; Microalgae; Valeur alimentaire; Crassostrea gigas; Tetraselmis suecica; Microalgues.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-6202.PDF
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Screening of marine algal extracts for anti-settlement activities against microalgae and macroalgae ArchiMer
Hellio, Claire; Berge, Jean-pascal; Beaupoil, Claude; Le Gal, Yves; Bourgougnon, Nathalie.
The ban on the use of TBT-based antifouling paints for boats under 25 m in length has lead to a search for new non-toxic antifoulants. One of the most promising alternative technologies to heavy metal based antifouling paint is the development of antifouling coatings whose active ingredients are naturally occurring compounds from marine organisms. This is based on the principle that marine organisms are also facing the problem of the presence of epibionts on their own surface. In this study, the antifouling activity of a series of aqueous, ethanolic and dichloromethane extracts from 30 algae from the North East Atlantic coast was investigated. The extracts were tested in laboratory assays against species representatives of two major groups of fouling...
Tipo: Text Palavras-chave: Antifouling; Antialgal; Microalgae; Macroalgae; Screening; Settlement; Spores.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/00057/16795/14304.pdf
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Valeur alimentaire de Tetraselmis striata et T. chui pour les larves de Crassostrea gigas ArchiMer
Ponis, Emanuele; Parisi, Guilina; Robert, Rene.
In an attempt to simplify mollusc hatchery-nursery procedures and to provide cost-effective alternative diets, the use of concentrated and preserved microalgae is an alternative to the use of fresh microalgae. Because of their ability for preservation, Prasinophytes appears to be good candidates for remote algal production. In the present work, the food value of fresh and preserved Tetraselmis chui and T. striata for umboned Crassostrea gigas larvae was studied. During the first experiment, fresh T. striata led to the best larval development, but these performances were not confirmed subsequently. When used as monospecific diets the food value of both Prasinophytes is unsufficient to support a good C. gigas larval development. However in bispecific diets a...
Tipo: Text Palavras-chave: Food value; Microalgae; Crassostrea gigas; Tetraselmis chui; Tetraselmis striata; Valeur alimentaire; Microalgues; Crassostrea gigas; Tetraselmis chui; Tetraselmis striata.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-6203.PDF
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Расчет объемов клеток микроводорослей и планктонных инфузорий Черного Моря IBSS Repository
Брянцева, Ю. В.; Курилов, А. В..
В данном пособии приводятся формулы для расчета объема клеток фитопланктона и планктонных инфузорий методом “истинного” объема. Впервые приводится список видов инфузорий, с указанием соответствующих фигур и поправочных коэффициентов. Для гидробиологов и экологов.
Tipo: Other Palavras-chave: Microalgae; Planktonic infusoria; Black Sea Cells Volume Phytoplankton Cells Volume Phytoplankton http://aims.fao.org/aos/agrovoc/c_1418 http://aims.fao.org/aos/agrovoc/c_8288 http://aims.fao.org/aos/agrovoc/c_13344.
Ano: 2003 URL: http://repository.ibss.org.ua/dspace/handle/99011/658
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Diets of tadpoles from a temporary pond in southeastern Brazil (Amphibia, Anura) Zoologia
Rossa-Feres,Denise de C.; Jim,Jorge; Fonseca,Mariluce Gonçalves.
The diet of tadpoles of 13 anuran species was determined to verify whether food resource partitioning occurs and whether the degree of diet similarity is related to taxonomic affinity. Tadpoles of all species studied were mainly herbivorous, except for these of Leptodactylus fuscus (Schneider, 1799) which were mycophagous. Although some species had exclusive items in their diet, most tadpole species ingested the same items, but differed in the amount of each item consumed. Two guilds were found: tadpoles that feed on diatoms on the pond bottom, and tadpoles that feed on Oedogonium Link, 1820 algae in midwater. Diet similarity was related to the taxonomic relationship, microhabitat and feeding behavior of tadpoles indicating that the community organization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Community ecology; Food partitioning; Microalgae.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000400003
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Urban secondary sewage: an alternative medium for the culture of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae) BABT
Costa,Rauquírio André Albuquerque Marinho da; Koening,Maria Luise; Macedo,Sílvio José de.
The effect of different concentrations (10, 20, 30 and 40%) of urban secondary sewage on the growth of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae) was examined to verify the possibility of its use as an alternative culture medium for these species. Stocks and 700mL duplicate batch cultures were maintained under controlled laboratory conditions. 30% and 40% additions were the most efficient for the growth of both species. Statistical analysis of the adjusted growth (cell.mL-1) and biomass (µg chlorophyll-a.mL-1) curves performed using the Chi-square test (p<0.05) demonstrated significant differences between the nutritive medium f/2 and the 40% addition used for these two species. The results suggested that some of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Culture; Urban secondary sewage; Alternative medium.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300016
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Uptake of cadmium by Pseudokirchneriella supcapitata BABT
Casiraghi,Magela Paula; Luporini,Samuel; Silva,Eduardo Mendes da.
In this work the microalgae Pseudokirchneriella supcapitata was used for the removal of the cadmium in liquids. The accumulations of metal ions by the alga occur in two stages: a very fast absorption (passive adsorption) proceeded by a slower absorption (activate absorption). A mathematical model based on the surface absorption and on the transport into the interior of the cellular membrane was developed. The simulation model kinetic parameters were experimentally obtained. Through the results observed, the mathematical model was shown to be suitable when compared to the experimental results, confirming the validation of the mathematical model.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Microalgae; Heavy metals; Mathematical model.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800019
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Etude de nouvelles cibles moleculaires de cancer bronchopulmonaire non a petites cellules pharmacomodulées par des substances originales naturelles et synthetiques ArchiMer
Moreau, Dimitri.
Still rare disease at the beginning of the 20th century, the lung cancer is at the origin of more than 6 % of the deaths in France currently. It occupies there the first rank of the deaths by cancer whereas France is regarded as a country at the average risk among the Western countries. The discovery of new anti-cancer treatments increasingly more effective and specific, is thus today of primary importance. The validation of the capacity of a molecule to being able to inhibit the cancerous proliferation, passes by several stages of tests starting with the description of an activity at the cellular level. One of the strategies used for the discovery of new compounds antiproliferativs, passes by an investigation of the bioactiv potential of a great number...
Tipo: Text Palavras-chave: Microalgae; Natural products; Apoptosis; Antiproliferativ; Gene expression; Regulatory RNA; Molecular targets; Bronchopulmonary cancer; Microalgues; Molécules naturelles; Apoptose; Antiprolifératif; Expression génique; ARN régulateur; Cibles moléculaires; Cancer bronchopulmonaire.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-2196.pdf
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Nutritional value of six Pavlovophyceae for Crassostrea gigas and Pecten maximus larvae ArchiMer
Ponis, Emanuele; Probert, I; Veron, Benoit; Le Coz, Jean-rene; Mathieu, Michel; Robert, Rene.
Four unidentified new strains of Pavlovophyceae [Pavlova sp. AC 250 (M), Pavlova sp. AC 248 (Psh), Pavlova sp. AC 251 (Pth), Pavlova sp. AC 538 (Psm)] and two known species [Pavlova pinguis (Ppi), Rebecca salina (ex Pavlova salina, Rsa)] were characterized (productivity, size, dry weight, ash, gross composition, fatty acids, sterols) and their nutritional value in bispecific diets were evaluated both on Crassostrea gigas and Pecten maximus larvae. All microalgae exhibited poor food value for C gigas larvae. P. pinguis and R. salina did not sustain any growth, like the control starved larvae. No exo-toxicity of P. pinguis and R. salina was detected. These two species were weakly ingested by C. gigas larvae, while the four other pavlovophytes were...
Tipo: Text Palavras-chave: Biochemical composition; Larval nutrition; Nutritional value; Microalgae.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1558.pdf
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New microalgae for the Pacific oyster Crassostrea gigas larvae ArchiMer
Ponis, Emanuele; Probert, Ian; Veron, Benoit; Mathieu, Michel; Robert, Rene.
The number of microalgae of high nutritional value actually available to mollusc hatcheries is limited to a few species and this represents a constraint for the industry. Fifteen microalgal species belonging to seven different taxonomic classes were selected from the Algobank-Caen microalgal culture collection of the University of Caen. An initial screening of these microalgae, based on their cytomorphological characteristics and growth performances, led to the selection of four species (Imantonia rotunda, Emiliania huxleyi, Pseudoisochrysis paradoxa and Diacronema vlkianum), which were then tested as food for Crassostrea gigas larvae. Two species (L rotunda and E huxleyi) were of poor food value for oyster larvae, while P paradoxa and D. vlkianum resulted...
Tipo: Text Palavras-chave: Biochemical composition; Larval nutrition; Mollusc hatchery; Microalgae.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1547.pdf
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Microbial community structure in the sea surface microlayer at two contrasting coastal sites in the northwestern Mediterranean Sea ArchiMer
Joux, Fabien; Agogue, Helene; Obernosterer, Ingrid; Dupuy, Christine; Reinthaler, Thomas; Herndl, Gerhard J.; Lebaron, Philippe.
In an attempt to compare the microbial community structure between the sea surface microlayer (SML) and subsurface waters (SSW), we determined the enrichment factors (EF: the ratio of abundance or activity in the SML to abundance or activity in SSW) of 13 biological parameters. Samples were taken at 2 contrasting coastal sites in the Mediterranean Sea, corresponding to a high (Barcelona, Spain) and low (Banyuls-sur-Mer, France) urbanized area. Principal component analysis showed that temporal variability was much higher at Barcelona than at Banyuls, and that the characteristics of the SML and SSW samples were more closely related at Barcelona. At both sites, the SML was weakly enriched in heterotrophic bacteria (on average 1.1-fold), Synechococcus spp. (on...
Tipo: Text Palavras-chave: Sea surface microlayer; Enrichment factor; Microbial structure; Virus-like particles; Bacteria; Microalgae; Nanoflagellates.
Ano: 2006 URL: https://archimer.ifremer.fr/doc/00243/35434/33961.pdf
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